| PDF : |  40111233.pdf |
| title(En) : | Antibacterial Activity of Hinokitiol against Methicillin-resistant Stapylococcus aureus (MRSA) |
| author(En) : | Toshihiro Okabe, Kouji Saito, Toru Fukui, Kazuso Iinuma |
| information : | Mokuzai Gakkaishi 40(11), 1233-1238 (1994)
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| assort : | Original Article |
| keywords : | hinokitiol, MRSA, MSSA, antibacterial activity, chelate compound. |
| summary(En) : | Testing was conducted to determine the effectiveness of hinokitiol against methicillin-resistant Staphylococcus aureus (MRSA), an important cause of hospital-acquired infection. The antibacterial testing, made in accordance with the standard method described by Japan Society of Chemotherapy, showed that hinokitiol has an excellent antibacterial effect above 100μg/ml for both MRSA and methicillin-sensitive S. aureus (MSSA).
The antibacterial effects of hinokitiol against MRSA appeared to be primarily a denaturalizing effect on the structural protein; however, a triplet ring was generated during testing using the method of the agar disk diffusion susceptibility test, and MRSA showed an increasing potential during the decrease in the active germ in a culture medium exposed to hinokitiol solution. This is supposed to be due to the differences of the antibacterial action under large and small concentrations of hinokitiol in the culture medium. Using a phase-contact microscope, the activity of hinokitiol against the bacteria was observed, and it was determined that Escherichia coli, gram-negative rod, burst and/or solidified, but MRSA, gram-positive cocci, showed no change other than swelling.
Hinokitiol is unstable under light and oxygen; therefore it is necessary to be stabilized by the formation of metal-hinokitiol chelate compounds to achieve a stable reaction effect. It was found that the antibacterial effect of metal-hinokitiol chelate compounds changes according to the types of metals. |
